SrasV1, Main, Exploration, bibRecord, 002822

Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice

Identifieur interne : 002822 ( Main/Exploration ); précédent : 002821; suivant : 002823

Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice

Auteurs : Kazuya Shirato [Japon] ; Madoka Maejima [Japon] ; Asuka Hirai [Japon] ; Yasushi Ami [Japon] ; Natsumi Takeyama [Japon] ; Kotaro Tsuchiya [Japon] ; Kouich Kusanagi [Japon] ; Tetsuo Nunoya [Japon] ; Fumihiro Taguchi [Japon]

Source :

Archives of Virology [ 0304-8608 ] ; 2010-12-01.

RBID : ISTEX:D5E3BA48C010EC6D50FF1930007DDA90DAE6AAC0

Descripteurs français

English descriptors

KwdEn :
- Adaptation, Biological, Amino Acid Substitution (genetics), Animals, Brain (virology), Cell Fusion, Cells, Cultured, Disease Models, Animal, Female, Membrane Glycoproteins (genetics), Mice, Mice, Inbred ICR, Porcine epidemic diarrhea virus (genetics), Porcine epidemic diarrhea virus (pathogenicity), Pregnancy, Serial Passage, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Virulence.
MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Amino Acid Substitution, Porcine epidemic diarrhea virus.
- pathogenicity : Porcine epidemic diarrhea virus.
- virology : Brain.
- Adaptation, Biological, Animals, Cell Fusion, Cells, Cultured, Disease Models, Animal, Female, Mice, Mice, Inbred ICR, Pregnancy, Serial Passage, Spike Glycoprotein, Coronavirus, Virulence.

Abstract

Abstract: Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.

Url:

https://api.istex.fr/ark:/67375/VQC-QXGSRC8S-K/fulltext.pdf

DOI: 10.1007/s00705-010-0790-1

Affiliations:

Le document en format XML

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<author><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name>
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<author><name sortKey="Maejima, Madoka" sort="Maejima, Madoka" uniqKey="Maejima M" first="Madoka" last="Maejima">Madoka Maejima</name>
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<author><name sortKey="Hirai, Asuka" sort="Hirai, Asuka" uniqKey="Hirai A" first="Asuka" last="Hirai">Asuka Hirai</name>
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<author><name sortKey="Ami, Yasushi" sort="Ami, Yasushi" uniqKey="Ami Y" first="Yasushi" last="Ami">Yasushi Ami</name>
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<author><name sortKey="Takeyama, Natsumi" sort="Takeyama, Natsumi" uniqKey="Takeyama N" first="Natsumi" last="Takeyama">Natsumi Takeyama</name>
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<author><name sortKey="Tsuchiya, Kotaro" sort="Tsuchiya, Kotaro" uniqKey="Tsuchiya K" first="Kotaro" last="Tsuchiya">Kotaro Tsuchiya</name>
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<author><name sortKey="Kusanagi, Kouich" sort="Kusanagi, Kouich" uniqKey="Kusanagi K" first="Kouich" last="Kusanagi">Kouich Kusanagi</name>
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<author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
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<term>Cell Fusion</term>
<term>Cells, Cultured</term>
<term>Disease Models, Animal</term>
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<term>Membrane Glycoproteins (genetics)</term>
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<term>Fusion cellulaire</term>
<term>Glycoprotéine de spicule des coronavirus</term>
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<term>Grossesse</term>
<term>Modèles animaux de maladie humaine</term>
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<term>Souris de lignée ICR</term>
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<term>Virus de la diarrhée porcine épidémique (génétique)</term>
<term>Virus de la diarrhée porcine épidémique (pathogénicité)</term>
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<term>Viral Envelope Proteins</term>
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<term>Porcine epidemic diarrhea virus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Substitution d'acide aminé</term>
<term>Virus de la diarrhée porcine épidémique</term>
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<term>Pregnancy</term>
<term>Serial Passage</term>
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<term>Cellules cultivées</term>
<term>Femelle</term>
<term>Fusion cellulaire</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Grossesse</term>
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<front><div type="abstract" xml:lang="en">Abstract: Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.</div>
</front>
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<name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
<name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
<name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
<name sortKey="Takeyama, Natsumi" sort="Takeyama, Natsumi" uniqKey="Takeyama N" first="Natsumi" last="Takeyama">Natsumi Takeyama</name>
<name sortKey="Tsuchiya, Kotaro" sort="Tsuchiya, Kotaro" uniqKey="Tsuchiya K" first="Kotaro" last="Tsuchiya">Kotaro Tsuchiya</name>
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Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice

Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice

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Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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